Role of Calcium and Calcium Channels in Progesterone Induced Acrosome Reaction in Caprine Spermatozoa
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چکیده
منابع مشابه
Role of Calcium and Calcium Channels in Progesterone Induced Acrosome Reaction in Caprine Spermatozoa
There are several physiological and pharmacological evidences indicating that opening of voltage dependent Ca channels play a critical role in induction of acrosome reaction in mammalian sperm. We determined the intracellular free Ca concentration in ejaculated goat sperm using a fluorescent, Ca-specific probe, Fura2/AM, after the suspension of sperm in KRB medium, capable of sustaining capacit...
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We evaluated the presence and role of internal calcium stores in human uncapacitated spermatozoa by determining the effects of two inhibitors of Ca2+ ATPase of the sarco-endoplasmic reticulum (SERCA-ATPase), thapsigargin and cyclopiazonic acid (CPA) on intracellular calcium concentrations, [Ca2+](i), plasma membrane potential and acrosome reaction. Using a fluorescent conjugate of thapsigargin,...
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The objectives of this study were: (i) to investigate the possible role of T-type Ca(2+) channels on the acrosome reaction (AR) of human spermatozoa; and (ii) to determine the sub-type of T-type calcium channels involved in the AR. The AR was induced in vitro by mannose-bovine serum albumin (BSA). The inhibitory effects of mibefradil (T-type Ca(2+) channel blocker), NiCl(2), or nifedipine (L-ty...
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نتایج این مطالعه نشان می دهد که مقدار کلسیم (یونیزه و تام)، منیزیم و فسفر به طور معنی داری تغییر پیدا کرد به جزء مقدار کلسیم تام و منیزیم در روش داخل عضلانی که تغییر معنی داری نداشته است. بر اساس نتایج این مطالعه می توان نتیجه گیری کرد که اکسی تتراساکلین بر روی مقادیر سرمی کلسیم و منیزیم تأثیر می گذارد باید مقدار این کاتیون ها در بیماران، در هنگامی که اکسی تتراسایکلین استفاده می شود مورد توجه ق...
15 صفحه اولZona pellucida and progesterone-induced Ca2+ signaling and acrosome reaction in human spermatozoa.
The mammalian acrosome reaction (AR) is the fusion, at multiple points, of the outer acrosomal membrane with the overlying plasma membrane. This fusion causes the release of acrosomal contents and the exposure of the inner acrosomal membrane. The time taken for this process to occur is not accurately known. Estimates for AR in mouse spermatozoa vary from ;2 minutes after stimulation with solubi...
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ژورنال
عنوان ژورنال: Asian-Australasian Journal of Animal Sciences
سال: 2002
ISSN: 1011-2367,1976-5517
DOI: 10.5713/ajas.2002.949